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GrainGenes Reference Report: PMB-23-287

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Reference	PMB-23-287
Title	The plant mitochondrial open reading frame orf221 encodes a membrane-bound protein
Journal	Plant Molecular Biology
Year	1993
Volume	23
Pages	287-295
Author	Prioli L Huang J Levings C, III
Abstract	We have shown that the open reading frame orf221 is an active mitochondrial gene which encodes a novel mitochondrial polypeptide The orf221 sequences common to higher plants but absent in animal and fungal mitochondria A mitochondrial polypeptide with an apparent molecular weight of 21 000 was detected with a polyclonal antibody raised against an ORF221 fusion protein In organello translation followed by immunoprecipitation with the anti-ORF221 antibody demonstrated that this polypeptide is encoded by the orf221 gene in plant mitochondria The ORF221 was found to be a mitochondrial membrane protein in normal (N), cms-T, and cms-C cytoplasms of several inbred lines of maize (Zea mays L. and in other plant species.
Keyword	[ Hide all but 1 of 11 ] cell-membranes immunocytochemistry membrane protein mitochondria mitochondrial dna mitochondrial-membranes nicotiana tabacum saccharomyces cerevisiae structural genes triticum aestivum zea mays

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