GrainGenes Reference Report: PMB-19-699
Reference
PMB-19-699
Title
A barley cDNA clone encoding a type III chlorophyll a-b-binding polypeptide of the light-harvesting complex II
Journal
Plant Molecular Biology
Year
1992
Volume
19
Pages
699-703
Author
Brandt J Nielsen VS Thordal-Christensen H Simpson DJ Okkels JS
Type
Article
Language
English
Abstract
The nucleotide sequence of a leaf cDNA clone encoding a Type III chlorophyll a/b-binding (CAB) protein of light-harvesting complex II (LCHII) in barley [Hordeum vulgare] is reported. Sequence comparisons and results from in vitro import into chloroplasts demonstrate that the cDNA clone encodes a functional transit peptide of 45 amino acid residues and a mature polypeptide of 223 residues with a predicted molecular mass of 24.3 kDa. After insertion into thylakoids, the mature protein is resistant to protease attack. Hybridization analysis using a gene-specific probe shows that the gene is expressed in dark-grown seedlings and that the amount of mRNA increases during illumination.
Probe
pCAB-3
Keyword
[ Hide all but 1 of 7 ] Complementary DNA DDBJ-X63197 EMBL-X63197 GENBANK-X63197 Hordeum vulgare Molecular sequence data Nucleotide sequence
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PMB-19-699
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||