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GrainGenes Reference Report: PMB-18-1151

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Reference	PMB-18-1151
Title	cDNA amino acid and carbohydrate sequence of barley seed-specific peroxidase bp 1
Journal	Plant Molecular Biology
Year	1992
Volume	18
Pages	1151-1161
Author	Johansson A Rasmussen SK Harthill JE Welinder KG
Type	Article
Language	English
Abstract	The major peroxidase of barley seed BP 1 was characterized. Previous studies showed a low carbohydrate content, low specific activity and tissue-specific expression, and suggested that this basic peroxidase could be particularly useful in the elucidation of the structure- function relationship and in the study of the biological roles of plant peroxidase (S.K. Rasmussen, K. G. Welinder and J. Hejgaard (1991) Plant Mol Biol 16: 317-327). A cDNA library was prepared from mRNA isolated from seeds 15 days after flowering. Full-length clones were obtained and showed 3' end length variants, a G+C content of 69% in the translated region, a 90% G or C preference in the wobble position of the codons and a typical signal peptide sequence. N-terminal amino acid sequencing and sequence analysis of tryptic peptides verified 98% of the sequence of the mature BP 1 which contains 309 amino acid residues. BP 1 is the first characterized plant peroxidase which is not blocked by proglutamate. BP 1 polymorphism was observed. BP 1 is less than 50% identical to other plant peroxidase which, taken together with its developmentally dependent expression in the endosperm 15-20 days after flowering, suggests a unique biological role of this enzyme. The barley peroxidase is processed at the C-terminus and might be targeted to the vacuole. The single site of glycosylation is located near the C-terminus in the N-glycosylation sequon -Asn-Cys-Ser- in which Cys forms part of a disulphide bridge. The major glycan is a typical plant modified-type structure, Man.alpha.1-6(Xyl.beta.1- 2)Man.beta.-1-4GlcNAc.beta.1-4(Fuc.alpha.1-3)GlcNAc. The BP 1 gene was RFLP-mapped on barley chromosome 3, and we propose Prx5 as the name for this new peroxidase locus.
Probe	pc13A9 pc59D9
Keyword	[ Hide all but 1 of 13 ] Carboxy-terminal Complementary DNA DDBJ-M73234 EMBL-M73234 GENBANK-M73234 Glycosylation Hordeum-vulgare Molecular sequence data Nucleotide sequence Processing Restriction fragment length polymorphism Signal peptide Targeting

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