GrainGenes Reference Report: BBR-214-1033
Reference
BBR-214-1033
Title
mRNA binding properties of wheat germ protein synthesis initiation factor 2
Journal
Biochemical and Biophysical Research Communications
Year
1995
Volume
214
Pages
1033-1039
Author
Benkowski L Ravel J Browning K
Abstract
The binding of protein synthesis initiation factor (eIF-) 2 to mRNA was measured by retention of the mRNA/eIF-2 complexes on nitrocellulose filters The binding of eIF-2 to mRNA was inhibited by GDP and GTP; the inhibition by GTP was enhanced by the presence of Met-tRNAi In addition, the formation of eIF-2/GDP binary complex and eIF-2/GTP/Met-tRNAi ternary complex was inhibited by mRNA These data indicate that mRNA binds to a site on eIF-2 that is the same or overlaps the site to which guanine nucleotides bind eIF-2 This finding strongly suggests that inhibition of ternary complex formation by mRNA may be the result of competition between mRNA and GTP and not competition between mRNA and Met-tRNAi.
Keyword
[ Hide all but 1 of 9 ] binding guanosine-diphosphate guanosine-triphosphate inhibition messenger rna necrovirus-group protein-synthesis satellite-tobacco-necrosis-necrovirus wheat germ
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: BBR-214-1033
|
| |||||||||||
|
| |||||||||||
|
| |||||||||||
|
| |||||||||||
|
| |||||||||||
|
| |||||||||||
|
| |||||||||||
|
| |||||||||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||