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GrainGenes Reference Report: PLC-76-147

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Reference	PLC-76-147
Title	Comparison of anther and microspore culture in the embryogenesis and regeneration of rye (Secale cereale)
Journal	Plant Cell
Year	2000
Volume	76
Pages	147-157
Author	Ma R Guo YD Pulli S
Abstract	Summary: Anther culture in solid and liquid medium and isolated microspore culture were compared in rye genotypes with potential agronomic characteristics. Some important factors influencing androgenic capacity were optimised. Three weeks cold pre-treatment of spikes and two days mannitol pre-treatment of anthers maximized callus and green plant yield in both culture methods. Intensity order of the culture methods in callus and green plant production was: isolated microspore culture, anther culture in liquid medium and anther culture in solid medium. Genotype ability of embryogenesis followed the same pattern in both cultivation methods. Kinetin (BA) with genotype dependent concentrations created the most effective regeneration conditions
Keyword	[ Hide all but 1 of 31 ] androgenesis anther anther culture anthers callus capacity cold concentration cooling cultivation culture media cultured cells dose response embryogenesis factor genetic variation haploidy in vitro regeneration kinetin mannitol media methodology micropropagation microspore pattern regeneration rye secale secale cereale spikes yield

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