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GrainGenes Reference Report: PLC-72-193

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Reference	PLC-72-193
Title	Callus induction and organogenesis in wheat/Aegilops longissima chromosome addition lines
Journal	Plant Cell
Year	2000
Volume	72
Pages	193-197
Author	Tyankova ND Zagorska NA Dimitrov B
Abstract	Summary: The influence of individual Aegilops longissima addition chromosomes on callus induction and organogenesis of in vitro cultivated immature embryos of wheat was studied. Immature 14-day-old embryos of seven Chinese Spring lines with added chromosomes of Aegilops longissima were in vitro cultivated. Although relatively high number of calli were formed in embryo cultures of all lines studied, the different genotypes expressed variation in respect to their response to in vitro cultivation. The number of initiated calli of line D, containing chromosome arms 7S1L and 4S1L was relatively low. The lines showed substantial differences in respect to the number of initiated morphogenic calli and regenerants recovered. The highest number of morphogenic calli and regenerants were obtained in cultures of lines containing the chromosomes 2S1, 6S1 and to less extend 3S1
Keyword	[ Hide all but 1 of 24 ] addition line aegilops aegilops longissima callus chinese chromosome chromosome addition chromosome addition lines cultivation culture media difference embryo embryo (plant) embryo culture genetic improvement genetic variation immature embryo in vitro regeneration induction methodology micropropagation morphogenesis organogenesis triticum aestivum

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