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GrainGenes Reference Report: PLC-63-35

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Reference	PLC-63-35
Title	Effects of free and chelated iron on in vitro androgenesis in barley and wheat
Journal	Plant Cell
Year	2000
Volume	63
Pages	35-40
Author	Novotny J Vagera J Ohnoutkova L
Abstract	Summary: A suitable form of iron supplement in the induction medium was found to be important for further development of induced pollen embryos in barley and wheat cultivars (genotypes), especially those providing few green plants via in vitro androgenesis. Genotypes able to regenerate many green plants were less susceptible to the lack of iron in induction medium. Although Fe-EDTA was found to be a suitable form of iron in the induction medium, androgenesis was also induced on media containing non-chelated iron (Fe2+ and Fe3+ ions). EDTA alone without iron inhibited the androgenic response even in the wheat cv. Florida, a model cultivar for androgenesis in wheat. In all barley cultivars under study including cv. Igri, a model cultivar for androgenesis in barley, EDTA alone caused an almost total suppression of androgenesis
Keyword	[ Hide all but 1 of 31 ] androgenesis anther culture barley barley cultivars cultivar culture media development dosage effects edta embryo ferric ions ferrous ions genetic variation genotype green hordeum vulgare in vitro in vitro androgenesis induction ion iron media methodology micropropagation model pollen regenerative ability suppression triticum aestivum vitro wheat cultivar

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