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GrainGenes Reference Report: PLC-15-271

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Reference	PLC-15-271
Title	The transcription factors HvABI5 and HvVP1 are required for the abscisic acid induction of gene expression in barley aleurone cells
Journal	Plant Cell
Year	2003
Volume	15
Pages	271-284
Author	Casaretto J Ho THD
Abstract	Summary: The abscisic acid (ABA) response promoter complexes (ABRCs) of the HVA1 and HVA22 genes have been shown to confer ABA-induced gene expression in cereals. A barley basic domain/Leu zipper (bZIP) transcription factor, HvABI5, is able to recognize ABRCs in vitro in a sequence-specific manner and to transactivate ABRC-beta-glucuronidase reporter genes when introduced to barley aleurone cells via particle bombardment. This transactivation is dependent on the presence of another transcription factor, HvVP1, and cannot be blocked by the negative regulator abi1-1. Using the double-stranded RNA interference technique, we show that HvABI5 and HvVP1 are necessary for the ABA induction of gene expression but have no effect on another hormone-regulated process, the gibberellin-induced and ABA-suppressed expression of alpha-amylase. Our work indicates that although other typical plant bZIP transcription factors may bind ABRCs in vitro, HvABI5 is related to a subfamily of bZIPs responsible for the ABA induction of gene expression. Furthermore, HvABI5 and HvVP1 are not involved in the ABA suppression of gene expression
External Databases	Pubmed: 12509536
Keyword	[ Hide all but 1 of 31 ] aba acid activator aleurone arabidopsis barley barley aleurone barley aleurone cells bombardment cell dna binding activity double stranded rna gene hva1 in vitro induction interference maize negative regulator particle bombardment plant bzip protein promoter regulator reporter signal transduction specification elements suppression transcription transcription factor viviparous-1 water-deficit

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