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GrainGenes Reference Report: PLC-14-537

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Reference	PLC-14-537
Title	An integrated physical and genetic map of the rice genome
Journal	Plant Cell
Year	2002
Volume	14
Pages	537-545
Author	Chen MS [ Show all 39 ]
Abstract	Summary: Rice was chosen as a model organism for genome sequencing because of its economic importance, small genome size, and syntenic relationship with other cereal species. We have constructed a bacterial artificial chromosome finger-print-based physical map of the rice genome to facilitate the whole-genome sequencing of rice. Most of the rice genome (approximately 90.6%) was anchored genetically by overgo hybridization, DNA gel blot hybridization, and in silico anchoring. Genome sequencing data also were integrated into the rice physical map. Comparison of the genetic and physical maps reveals that recombination is suppressed severely in centromeric regions as well as on the short arms of chromosomes 4 and 10. This integrated high-resolution physical map of the rice genome will greatly facilitate whole-genome sequencing by helping to identify a minimum tiling path of clones to sequence. Furthermore, the physical map will aid map-based cloning of agronomically important genes and will provide an important tool for the comparative analysis of grass genomes
External Databases	Pubmed: 11910002
Keyword	[ Hide all but 1 of 34 ] arms artificial chromosome bacterial bacterial artificial chromosome blot hybridization centromeric regions chromosome clone cloning dna gene genetic map genome genome sequencing genome size grass genomes hybridization map map-based cloning marker model organism physical map physical maps population recombination region rice rice genome sequence sequencing short arms small genome tool

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