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GrainGenes Reference Report: PLC-11-1867

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Reference	PLC-11-1867
Title	Tonoplast intrinsic protein isoforms as markers for vacuolar functions
Journal	Plant Cell
Year	1999
Volume	11
Pages	1867-1882
Author	Jauh G Phillips T Rogers J
Abstract	Plant cell vacuoles may have storage or lytic functions, but biochemical markers specific for the tonoplasts of functionally distinct vacuoles are poorly defined Here, we use antipeptide antibodies specific for the tonoplast intrinsic proteins alpha-TIP, gamma-TIP, and delta-TIP in confocal immunofluorescence experiments to test the hypothesis that different TIP isoforms may define different vacuole functions Organelles labeled with these antibodies were also labeled with antipyrophosphatase antibodies, demonstrating that regardless of their size, they had the expected characteristics of vacuoles Our results demonstrate that the storage vacuole tonoplast contains delta-TIP, protein storage vacuoles containing seed-type storage proteins are marked by alpha- and delta- or alpha- and delta- plus gamma-TIP, whereas vacuoles storing vegetative storage proteins and pigments are marked by delta-TIP alone or delta- plus gamma-TIP In contrast, those marked by gamma-TIP alone have characteristics of lytic vacuoles, and results from other researchers indicate that alpha-TIP alone is a marker for autophagic vacuoles In root tips, relatively undifferentiated cells that contain vacuoles labeled separately for each of the three TIPs have been identified These results argue that plant cells have the ability to generate and maintain three separate vacuole organelles, with each being marked by a different TIP, and that the functional diversity of the vacuolar system may be generated from different combinations of the three basic types.
Keyword	[ Hide all but 1 of 12 ] biochemical-markers cotyledons hordeum vulgare immunoblotting immunocytochemistry lectins pisum sativum plant proteins pyrophosphatases root-tips tonoplast vacuole

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