GrainGenes Reference Report: PLC-11-1853
Reference
PLC-11-1853
Title
A two-component Enhancer-inhibitor transposon mutagenesis system for functional analysis of the Arabidopsis genome
Journal
Plant Cell
Year
1999
Volume
11
Pages
1853-1866
Author
[ Hide all but 1 of 6 ] Speulman E Metz P Arkel G Lintel-Hekkert B Stiekema W Pereira A
Abstract
A modified Enhancer-Inhibitor transposon system was used to generate a series of mutant lines by single-seed descent such that multiple I insertions occurred per plant The distribution of original insertions in the population was assessed by isolating transposon-flanking DNA, and a database of insertion sites was created Approximately three-quarters of the identified insertion sites show similarity to sequences stored in public databases, which demonstrates the power of this regimen of insertional mutagenesis To isolate insertions in specific genes, we developed three-dimensional pooling and polymerase chain reaction strategies that we then validated by identifying mutants for the regulator genes APETALA1 and SHOOT MERISTEMLESS. The system then was used to identify inserts in a class of uncharacterized genes involved in lipid biosynthesis; one such insertion conferred a fiddlehead mutant phenotype.
Keyword
arabidopsis thaliana
[ Show all 12 ]
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PLC-11-1853
|
| ||||||||
|
| ||||||||
|
| ||||||||
|
| ||||||||
|
| ||||||||
|
| ||||||||
|
| ||||||||
|
| ||||||||
|
|
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||