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GrainGenes Reference Report: PLC-7-499

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Reference	PLC-7-499
Title	Molecular responses to abscisic acid and stress are conserved between moss and cereals
Journal	Plant Cell
Year	1995
Volume	7
Pages	499-506
Author	Knight C [ Show all 10 ]
Abstract	Promoter elements from the wheat Em gene have been characterized These elements are inducible by abscisic acid (ADA) and by osmotic stress. In this study we demonstrated that the same promoter elements function in a distantly related plant species the moss Physcomitrella patens. Transient and stable expression of the beta-glucuronidase reporter gene was used to determine that the heterologous wheat promoter also responds to osmotic stress and ABA in moss. Mutational analysis of the promoter indicated that the mechanism of gene regulation is conserved in both species. Gel retardation and DNase I footprint analyses were conducted to characterize further the interaction of moss transcription factors with the Em promoter. In addition the synthesis of stress-related polypeptides in moss was observed. The evolutionary significance of these data and the potential for studying the entire ABA perception-response pathway in moss are discussed.
Keyword	[ Hide all but 1 of 21 ] abscisic acid beta-glucuronidase bryophyta dna binding-proteins em-promoter gene expression genetic regulation genetic transformation mannitol mosses osmotic-pressure osmotic-stress physcomitrella-patens promoters recombinant-dna reporter genes stress transcription transcription-factors transgenic-plants triticum aestivum

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