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GrainGenes Reference Report: PLA-218-886

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Reference	PLA-218-886
Title	Regulation of alpha-galactosidase gene expression in primary foliage leaves of barley (Hordeum vulgare L.) during dark-induced senescence
Journal	Planta
Year	2004
Volume	218
Pages	886-889
Author	Chrost B Daniel A Krupinska K
Abstract	Summary: (alpha)-Galactosidase activity ((alpha)-D-galactoside galactohydrolase, EC 3.2.1.22) increased during dark-induced senescence in primary foliage leaves of barley (Hordeum vulgare L. cv. Steffi). The changes in activity were accompanied by parallel changes in expression of the HvSF11 gene encoding a putative (alpha)-galactosidase. The transcript level of HvSF23 encoding a second putative (alpha)-galactosidase stayed constant during leaf senescence. Both (alpha)-galactosidase activity and the level of the HvSF11 transcript decreased after exogenous application of sucrose and glucose to detached dark-incubated leaves. In contrast, the HvSF23 transcript level was not influenced by sugars. Application of glucose analogs to detached and dark-incubated leaves revealed that phosphorylation of hexose by hexokinase modulates both the (alpha)-galactosidase activity and the expression of HvSF11. These results indicate that the expression of the genes coding for two (alpha)-galactosidase isoenzymes is regulated by different signalling pathways, suggesting different functions for the two gene products
External Databases	Pubmed: 14652758
Keyword	[ Hide all but 1 of 29 ] alpha-galactosidase (enzymes,cdnas) analogs arabidopsis thaliana barley dark-induced senescence foliage gene glucose hordeum hordeum vulgare inducible genes isoenzyme leaf senescence leaf senescence (barley) metabolism phosphorylation primary foliage leaves products purification regulation senescence signalling pathways source transition starvation stress sucrose sugar sugar-mediated gene repression (hexokinase) transcript

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