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GrainGenes Reference Report: PLA-218-261

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Reference	PLA-218-261
Title	Cloning and characterization of the granule-bound starch synthase II gene in rice: gene expression is regulated by the nitrogen level, sugar and circadian rhythm
Journal	Planta
Year	2003
Volume	218
Pages	261-268
Author	Dian W Jiang H Chen Q Liu F Wu P
Abstract	Summary: A full-length coding domain sequence of a gene analogous to granule-bound starch synthase (GBSS; ADP-glucose-starch glucosyltransferase, EC 2.4.1.21) was cloned and defined as OsGBSSII based on a Nitrogen (N)-starvation-induced cDNA library constructed using the rapid subtraction hybridization method. The deduced amino acid sequence of OsGBSSII was 62-85% identical to those of GBSS proteins from other plant species. The exon/intron organization of OsGBSSII was similar to that of OsGBSSI. OsGBSSII was mainly expressed in leaves and its protein was exclusively bound to starch granules in rice leaves, which suggests that the amylose in rice leaves is synthesized by OsGBSSII. N-starvation-induced expression of OsGBSSII could be repressed by supplying nitrate, ammonia or amino acid (glutamic acid or glutamine), glucosamine (an inhibitor of hexokinase) or dark conditions. These results indicate that N-starvation induction was dependent on the photosynthetic product and hexokinase in rice leaves. Sugars induced the accumulation of OsGBSSII transcripts in excised leaves through glycolysis-dependent pathways. OsGBSSII gene expression is regulated by the circadian rhythm in rice leaves
Keyword	[ Hide all but 1 of 45 ] amino acid amino acid sequence ammonium amylose cdna cdna library circadian rhythm cloning complementary dna culture media domain escherichia coli excised leaves exons gene gene expression gene expression regulation glutamine grain crops granule granule-bound starch synthase granules hexokinase hybridization induction inhibitor introns library nitrate nitrogen organization oryza sativa plant proteins rice rice leaves sequence sequence alignment sequence analysis starch starch granules starch synthase starches sugar synthase tissue distribution

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