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GrainGenes Reference Report: PLA-215-727

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Reference	PLA-215-727
Title	A germination-related gene encoding a serine carboxypeptidase is expressed during the differentiation of the vascular tissue in wheat grains and seedlings
Journal	Planta
Year	2002
Volume	215
Pages	727-734
Author	Dominguez F Gonzalez MC Cejudo FJ
Abstract	Summary: Carboxypeptidases expressed in the aleurone layer participate in the mobilization of endosperm storage proteins during cereal grain germination. The genes encoding these proteins are also expressed in the scutellum of germinating grains, but their function in this organ is not yet clear. We have analyzed the expression of a carboxypeptidase III (CPIII) gene in germinating wheat (Triticum aestivum L.) grains. CPIII transcripts accumulated transiently in the scutellum showing a maximum at 2-3 days after imbibition and were exclusively localized to the scutellar vascular tissue. The analysis of CPIII expression in developing shoots and roots from growing seedlings confirmed the localization of CPIII transcripts to differentiating vascular tissue. The TUNEL assay detected in situ nuclear DNA fragmentation in cells showing CPIII expression, indicating that they undergo programmed cell death. Relative RT-PCR analysis showed that the CPIII gene expressed at high level in aleurone cells is the one expressed in vegetative tissues, and allowed the use of this gene as a molecular marker of tracheary element differentiation in wheat seedlings. These results are indicative of the involvement of serine carboxypeptidases in programmed cell death during the development of the vascular tissue in wheat, a new role for these enzymes, besides the mobilization of starchy-endosperm proteins during germination
External Databases	Pubmed: 12244437
Keyword	[ Hide all but 1 of 27 ] acid carboxypeptidases aleurone aleurone layers alpha-amylase barley grains cell cereal-grains dna endosperm gene germination gibberellic acid in situ localization marker programmed cell death seed seedling serine protease storage storage proteins tissue tracheary element tracheary element differentiation triticum triticum aestivum wheat seedlings

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