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GrainGenes Reference Report: PLA-215-319

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Reference	PLA-215-319
Title	Molecular analysis of the barley (Hordeum vulgare L.) gene encoding the protein kinase PKABA1 capable of suppressing gibberellin action in aleurone layers
Journal	Planta
Year	2002
Volume	215
Pages	319-326
Author	Yamauchi D Zentella R Ho THD
Abstract	Summary: Abscisic acid (ABA) induces the expression of a gene encoding a protein kinase, PKABA1, in wheat (Triticum aestivum L.). Transient expression of the cDNA encoding this kinase suppresses the gibberellin (GA)-induced expression of alpha-amylase genes in barley (Hordeum vulgare L.) aleurone layers. We have cloned the cDNA and genomic sequences for the barley PKABA1 (HvPKABA1) to further study its role in regulating the GA-inducible gene expression in barley seeds. The amino acid sequence deduced from the cDNA shows 97% identity with that of wheat PKABA1. RNA blot analysis indicated that ABA induced, but GA suppressed, the expression of HvPKABA1 in barley seeds. Transient expression of HvPKABA1 also suppressed the GA-inducible alpha-amylase gene expression. These results suggest that HvPKABA1 is probably involved in the signal transduction pathway from ABA to suppress the GA-inducible gene expression. Southern blot analysis showed that HvPKABA1 is likely a single-copy gene in barley. The sequence of the genomic clone reveals that HvPKABA1 consists of eight introns, the first one being more than 6 kb long. The mapped transcription start site of this gene suggests that it may have a TATA-less promoter
Keyword	[ Hide all but 1 of 46 ] aba aleurone aleurone layer aleurone layers alpha-amylase gene amino acid amino acid sequence barley barley seed canavalia gladiata cdna clone concanavalin-a ga gene gene expression gene structure and function genomic clone genomic sequence gibberellin hordeum intron kb kinase layer layers messenger rna molecular analysis pathway pkaba1 promoter protein sequence signal signal transduction signal transduction pathway single-copy snf1 transcription transcription start site transduction transduction pathway transient transient expression triticum triticum aestivum

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