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GrainGenes Reference Report: PLA-214-813

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Reference	PLA-214-813
Title	The sugary-type isoamylase in wheat: tissue distribution and subcellular localisation
Journal	Planta
Year	2002
Volume	214
Pages	813-820
Author	Genschel U Abel G Lorz H Lutticke S
Abstract	Summary: To gain an increased understanding of the role of isoamylase (EC 3.2.1.68) in amylopectin synthesis, we studied the tissue-specific distribution and subcellular localisation of this enzyme in wheat (Triticum aestivum L.). A cDNA for wheat isoamylase was isolated from an endosperm-specific library and the missing 5' end was amplified by anchored polymerase chain reaction. Isoamylase transcripts were detected in reproductive and vegetative tissues, with the highest levels occurring in developing kernels. Wheat kernels were then dissected into embryo, endosperm, pericarp and chlorophyll layer, and subjected to protein blot analysis. Isoamylase was most abundant in the endosperm. Within the endosperm, the vast majority of isoamylase was soluble. A much smaller amount of the enzyme was associated with starch granules. Isoamylase was not trapped within starch granules and was absent from dry seeds. Isoamylase was also present in green tissue, which suggests a role in the synthesis of both reserve and leaf starches
Keyword	[ Hide all but 1 of 32 ] amino acid sequence amylopectin biosynthesis cdna chain chlorophyll complementary dna embryo enzyme granule isoamylase kernel layer localisation messenger rna nucleotide sequence pericarp plant embryos polymerase polymerase chain reaction protein seed seed development starch starch granule starches synthesis tissue triticum triticum aestivum vegetative tissues wheat kernel

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