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GrainGenes Reference Report: PLA-213-101

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Reference	PLA-213-101
Title	Amitrole treatment of etiolated barley seedlings leads to deregulation of tetrapyrrole synthesis and to reduced expression of Lhc and RbcS genes
Journal	Planta
Year	2001
Volume	213
Pages	101-108
Author	La Rocca N Rascio N Oster U Rudiger W
Abstract	Summary: The effect of amitrole, known as an inhibitor of carotenoid biosynthesis, upon tetrapyrrole biosynthesis and its regulation has been studied. Etiolated barley (Hordeum vulgare L.) seedlings, grown in 125 micromolar amitrole, accumulated high levels of 5-aminolevulinate, Mg-protoporphyrin, Mg-protoporphyrin monomethyl ester, and protochlorophyllide. The amitrole-treated seedlings did not form paracrystalline prolamellar bodies, and the induction of Lhc and RbcS gene expression was reduced by non-photooxidative, low-intensity light. None of these events was observed upon treatment of the seedlings with 100 micromolar norflurazon, another inhibitor of carotenoid biosynthesis. The effect of amitrole cannot be explained solely by interaction with a presumed feedback inhibition of 5-aminolevulinate synthesis since incubation with amitrole and 5-aminolevulinate indicated that deregulation also occurs at later steps of tetrapyrrole biosynthesis. A possible relationship between this deregulation and ultrastructural changes is discussed. In connection with previously published data, we discuss Mg-protoporphyrin and its monomethyl ester as possible candidates for a 'plastid signal' that operates as a negative factor, reducing the expression of Lhc and RbcS genes in this higher plant
Keyword	[ Hide all but 1 of 40 ] 5-aminolevulinate a-b protein amitrole barley barley seedlings biosynthesis bodies carotenoid biosynthesis chlorophyll precursors cress seedlings enzyme levels ester etiolated barley etioplast structure events feedback gene gene expression greening process hordeum hordeum vulgare induction inhibition inhibitor interaction its lead light magnesium photoporphyrin norflurazon photodynamic herbicides photooxidative destruction plastid signal prolamellar bodies prolamellar body protochlorophyllide rbcs genes regulation seedling synthesis

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