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GrainGenes Reference Report: PLA-212-864

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Reference	PLA-212-864
Title	In vivo evidence that Ids3 from Hordeum vulgare encodes a dioxygenase that converts 2 '-deoxymugineic acid to mugineic acid in transgenic rice
Journal	Planta
Year	2001
Volume	212
Pages	864-871
Author	Kobayashi T [ Show all 6 ]
Abstract	Summary: We proposed that an Fe-deficiency-induced gene, Ids3 (Iron deficiency specific clone no. 3), from barley (Hordeum vulgare L.) roots encodes a dioxygenase that catalyzes the hydroxylation step from 2'-deoxymugineic acid (DMA) to mugineic acid (MA). To prove this hypothesis, we introduced the Ids3 gene into rice (Oryza sativa L.), which lacks Ids3 homologues and secretes DMA, but not MA. Transgenic rice plants, carrying either Ids3 cDNA or a barley genomic DNA fragment (20 kb) containing Ids3, were obtained using Agrobacterium-mediated transformation. Ids3 cDNA under the control of the cauliflower mosaic virus 35S promoter was constitutively expressed in both the roots and the leaves of the transgenic rice, regardless of Fe nutrition status. In contrast, in the roots of transformants carrying a barley genomic fragment, transcripts of Ids3 were markedly increased in response to Fe deficiency. Slight expression of Ids3 was also observed in the leaves of the Fe-deficient plants. Western blot analysis confirmed the induction of Ids3 in response to Fe deficiency in the roots of the transformants carrying a genomic fragment. These expression patterns indicate that the 5'-flanking region of Ids3 works as a strong Fe-deficiency-inducible promoter in rice, as well as in barley. Both kinds of transgenic rice secreted MA in addition to DMA under Fe-deficient conditions, but wild-type rice secreted only DMA. This is in vivo evidence that IDS3 is the 'MA synthase' that converts DMA to MA
External Databases	Pubmed: 11346963
Keyword	[ Hide all but 1 of 48 ] 35s promoter addition lines agrobacterium-mediated transformation barley barley roots cauliflower cdna clone deficiency deficiency-specific cdna dioxygenase dna expression patterns fe deficiency fe nutrition status gene genomic dna gramineous plants hordeum vulgare hydroxylation hypothesis id in vivo induction iron iron-deficiency methionine mosaic virus mt domains mugineic acid mugineic acid family phytosiderophores mugineic acid synthase nicotiana ine synthase nutrition oryza (transgenic) phytosiderophore production plant science promoter rice rice plant root transcript transformation transgenic transgenic rice plants virus western blot analysis wild-type

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