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GrainGenes Reference Report: PLA-210-188

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Reference	PLA-210-188
Title	Characterization of two cDNA clones encoding isoenzymes of the F1F0-ATPase inhibitor protein of rice mitochondria
Journal	Planta
Year	2000
Volume	210
Pages	188-194
Author	Nakazono M Imamura T Tsutsumi N Sasaki T Hirai A
Abstract	Summary: Two cDNA clones encoding F1F0-ATPase inhibitor proteins, which are loosely associated with the F1 part of the mitochondrial F1F0-ATPase, were characterized from rice (Oryza sativa L. cv. Nipponbare). A Northern hybridization showed that the two genes (designated as IF(1)-1 and IF(1)-2) are transcribed in all the organs examined. However, the steady-state mRNA levels varied among organs. A comparison of the deduced amino acid sequences of the two IF(1) genes and the amino acid sequence of the mature IF1 protein from potato revealed that IF(1)-1 and IF(1)-2 have N-terminal extensions with features that are characteristic of a mitochondrial targeting signal. To determine the subcellular localization of the gene products, the IF(1)-1 or IF(1)-2 proteins were fused in frame to the green fluorescent protein (GFP) or the fused GFP-beta-glucuronidase, and expressed transiently in onion or dayflower epidermal cells. Localized fluorescence was detected in mitochondria, confirming that the two IF(1) proteins are targeted to mitochondria
Keyword	[ Hide all but 1 of 40 ] adenosinetriphosphatase amino acid amino acid sequence animal proteins cdna clone cell complementary dna dna hybridization enzyme inhibitors epidermal-cells epidermis extension features fluorescence gene gene expression gene product gfp green fluorescent protein hybridization inhibitor inhibitor protein isoenzyme jellyfish localization messenger rna mitochondria mrna northern onion oryza sativa potato products pyrophosphatases rice sequence signal subcellular-localization transcription transgenic plant

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