GrainGenes Reference Report: PJB-36-595
Reference
PJB-36-595
Title
Application of random amplified polymorphic DNA (RAPD) technique for the identification of markers linked to salinity tolerance in wheat ( Triticum aestivum L.)
Journal
Pakistan Journal of Botany
Year
2004
Volume
36
Pages
595-602
Author
Mehboob uR Malik TA Chowdhary MA Iqbal MJ Yusuf Z
Abstract
The DNA marker for the low Na uptake trait of hexaploid wheat ( Triticum aestivum ) was identified. Individual plants from the F SUB 3 population segregating for salt tolerance and the parents (LU-26S and Rohtas-90) were grown in polyethylene tubes under saline conditions (electrical conductivity of 25 dS m SUP -1 ) and screened for K:Na ratio, chloride ions and net photosynthesis at the fourth leaf stage. The plants were then transplanted into pots filled with 7 kg of fertile soil and supplied with optimum amounts of water and nutrients until maturity. The correlations of K:Na and net photosynthesis with yield components (plant height, spike length, number of spikelets, number of grains and grain weight per spike) were calculated. Genomic DNA from 15 tolerant and 15 sensitive F SUB 3 plants was extracted. Bulked segregant analysis was used in the random amplified polymorphic DNA (RAPD) technique. DNA polymorphisms were observed using 148 primers. The primer OPZ-10 amplified a 680-bp polymorphic DNA fragment which linked to the K:Na ratio trait. This DNA fragment can be used for marker-assisted selection for breeding of salt-tolerant wheat. K:Na ratio and net photosynthesis were not correlated with the yield components
Keyword
GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture.
GrainGenes Reference Report: PJB-36-595
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GrainGenes is a product of the Agricultural Research Service of the US Department of Agriculture. | |||