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GrainGenes Reference Report: PHY-94-520

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Reference	PHY-94-520
Title	Genetic mapping of pathogenicity and aggressiveness of Gibberella zeae (Fusarium graminearum) toward wheat
Journal	Phytopathology
Year	2004
Volume	94
Pages	520-526
Author	Cumagun CJR Bowden RL Jurgenson JE Leslie JF Miedaner T
Abstract	Summary: Gibberella zeae is the major fungal pathogen of Fusarium head blight of wheat and produces several mycotoxins that are harmful to humans and domesticated animals. We identified loci associated with pathogenicity and aggressiveness on an amplified fragment length polymorphism based genetic map of G. zeae in a cross between a lineage 6 nivalenol producer from Japan and a lineage 7 deoxynivalenol producer from Kansas. Ninety-nine progeny and the parents were tested in the greenhouse for 2 years. Progeny segregated qualitatively (61:38) for pathogenicity:nonpathogenicity, respectively. The trait maps to linkage group IV, which is adjacent to loci that affect colony pigmentation, perithecium production, and trichothecene toxin amount. Among the 61 pathogenic progeny, the amount of disease induced (aggressiveness) varied quantitatively. Two reproducible quantitative trait loci (QTL) for aggressiveness were detected on linkage group I using simple interval analysis. A QTL linked to the TRI5 locus (trichodiene synthase in the trichothecene pathway gene cluster) explained 51% of the variation observed, and a second QTL that was 50 centimorgans away explained 29% of the phenotypic variation. TRI5 is tightly linked to the locus controlling trichothecene toxin type. The two QTLs, however, were likely part of the same QTL using composite interval analysis. Progeny that produced deoxynivalenol were, on average, approximately twice as aggressive as those that produced nivalenol. No transgressive segregation for aggressiveness was detected. The rather simple inheritance of both traits in this interlineage cross suggests that relatively few loci for pathogenicity or aggressiveness differ between lineage 6 and 7
Keyword	[ Hide all but 1 of 59 ] aflp cereal aggressiveness amplified fragment length polymorphism blight cluster culmorum deoxynivalenol differ disease diversity fusarium fusarium graminearum fusarium head blight gene gene-cluster genetic map genetic mapping gibberella zeae graminearum greenhouse head blight head-blight human humans infection inheritance interval japan kansas lineage linkage map mapping mycotoxin mycotoxins nivalenol pathogen pathogenicity pathway phenotypic variation pigmentation polymorphism population qtl qtls quantitative trait quantitative trait loci reduced virulence scab segregation synthase toxin trait loci transgressive segregation trichothecene trichothecene mycotoxins trichothecenes triticum aestivum winter rye

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