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GrainGenes Reference Report: PHY-92-710

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Reference	PHY-92-710
Title	Resistance to scald (Rhynchosporium secalis) in barley (Hordeum vulgare) studied by near-isogenic lines: I. Markers and differential isolates
Journal	Phytopathology
Year	2002
Volume	92
Pages	710-720
Author	[ Hide all but 1 of 8 ] Bjornstad A Patil V Tekauz A Maroy AG Skinnes H Jensen A Magnus H MacKey J
Abstract	Summary: Near-isogenic lines (NILs) with resistance for scald in seventh generation backcross with 'Ingrid' as recurrent parent (RP) were tested with seven differential isolates of Rhynchosporium secalis in Norway and Canada. NILs of 'Turk', 'Brier', 'CI 8162', 'La Mesita', 'Hispont', 'Atlas 46', 'Modoc', 'Hudson', 'Abyssinian', 'Steudelli', and 'CI 2222' also were evaluated for field reactions. The genetic characterization of the NILs (degree of isogeneity with Ingrid) and with each other was carried out. The molecular marker pattern shows that the backcrossing program has resulted in from 86.3 to 100% RP genome in the NILs, depending on the marker system. On an average, 96% RP genome was found in the NILs. There were certain consistent (pairwise) differences between the NILs and RP on chromosomes 3H and 7H. Both chromosomes are known to contain loci conferring resistance to R. secalis, indicating successful introgression from the donors into the NILs. Approximately two-thirds of the observed RP-NIL polymorphisms were linked to the assumed resistance in the NIL. Based on the marker and phenotypic analyses of the NILs, suggestions for a more appropriate and updated terminology of genes for resistance to R. secalis in barley are made. The proposed changes in nomenclature also indicate the differentials that are available as NILs and those lacking
Keyword	backcrossing [ Show all 36 ]

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