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GrainGenes Reference Report: AAR-52-1409

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Reference	AAR-52-1409
Title	Development of robust PCR-based DNA markers for each homoeo-allele of granule-bound starch synthase and their application in wheat breeding programs
Journal	Australian Journal of Agricultural Research
Year	2001
Volume	52
Pages	1409-1416
Author	McLauchlan A [ Show all 13 ]
Abstract	The absence of expression of the granule-bound starch synthase I (GBSSI) allele from chromosome 4A of wheat is associated with improved starch quality for making Udon noodles. Several PCR-based methods for the analysis of GBSS alleles have been developed for application in wheat. A widely applied approach has involved a simple PCR followed by electrophoretic separation of DNA products on agarose gels. The PCR amplifies one band from each of the loci on chromosomes 4A (Wx-B1), 7A (Wx-A1), and 7D (Wx-D1), and the band from the Wx-B1 locus is diagnostic for the occurrence of the null Wx-B1 allele that is associated with improved starch quality. The reliable detection of the null Wx-B1 allele has been important in identifying wheat breeding lines. Allele-specific PCR has also been used to successfully detect the occurrence of the null Wx-B1 allele. In the present paper the various protocols were evaluated by testing a segregating double haploid population from a cross between Cranbrook and Halberd and the tests gave good agreement in different laboratories. The application of the DNAbased tests applied in wheat breeding programs provides one of the first examples of a molecular marker selection for a grain quality trait being successfully applied in an Australian wheat breeding program.
External Databases	https://doi.org/10.1071/AR01036
Gene Class	Starch synthase Waxy proteins
Keyword	[ Hide all but 1 of 48 ] allele alleles australian wheat breeding breeding lines breeding programs chromosome chromosome 4a cross detection diagnostic dna dna marker gbssi gels grain quality granule bound starch synthase granule-bound starch synthase haploid loci locus marker marker-assisted selection method microsatellite molecular marker noodle quality paper pcr population products program programs protein protocol quality selection separation starch starch synthase starches synthase synthase-i trait triticum aestivum waxy waxy genes wheat breeding
Summarized in	Dubcovsky J MAS Wheat. Bringing Genomics to the Wheat Fields.Starch properties. Waxy mutants MAS Wheat. Marker Assisted Selection in Wheat.

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