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GrainGenes Marker Report: k10125

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Locus	k10125
Type	CAPS
Chromosome	7H
Map	Hv-HNxH602-7H 231
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	bast60C0105
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = FokI SNP = A/G SNP structure (UNICODE) = CAGCCGCCTCRTCCAGCGCCC Parent 1 SNP sequence = CAGCCGCCTCATCCAGCGCCC Parent 2 SNP sequence = CAGCCGCCTCGTCCAGCGCCC Recognized sequence for CAPs locus = NNNNNNNNNNNNNCATCC
Probe for Locus	bast60C0105
Locus	k10125
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = proteaseI (pfpI)-like protein (YLS5) [Arabidopsis thaliana] gb AAC79625.2 expressed protein [Arabidopsis thaliana]
Type	PCR
PCR primers	Left 5' - ATCGGAAGCCCCTTGTAAGT Right 5' - GAGTTTCTCGCAAGGACAGG
Sequence	BQ757627
PCR size	195