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GrainGenes Marker Report: k10012

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Locus
k10012
Type
CAPS
Chromosome
5H
Map
Hv-HNxH602-5H117.8
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
bast62E0610
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = HhaI
SNP = T/C
SNP structure (UNICODE) = CATGAGTGCGYTGTGTCCTGA
Parent 1 SNP sequence = CATGAGTGCGTTGTGTCCTGA
Parent 2 SNP sequence = CATGAGTGCGCTGTGTCCTGA
Recognized sequence for CAPs locus = GCGC
SNP = A/G
SNP structure (UNICODE) = NCANGACACARCGCANNNNRG
Parent 1 SNP sequence = TCANGACACAACGCACCNNAG
Parent 2 SNP sequence = NCANGACACAGCGCANNGGGG



Probe for Locus
bast62E0610
Locus
k10012
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = aleurain (EC 3.4.22.-) precursor - barley
Type
PCR
PCR primers
Left 5' - TCCGAGCACACGAACACTAC
Right 5' - CGACTAATGAGGGAAGGCAG
Sequence
BJ547125
PCR size
321