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GrainGenes Marker Report: k08669

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Locus	k08669
Type	CAPS
Chromosome	2H
Map	Hv-HNxH602-2H 49.9
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	BaH35F01
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = HaeIII SNP = G/C SNP structure (UNICODE) = CGGCGTAGGCSAGCTTGTCGT Parent 1 SNP sequence = CGGCGTAGGCGAGCTTGTCGT Parent 2 SNP sequence = CGGCGTAGGCCAGCTTGTCGT Recognized sequence for CAPs locus = GGCC
Probe for Locus	BaH35F01
Locus	k08669
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = mycolic acid methyl transferase-like protein [Arabidopsis thaliana]
Type	PCR
PCR primers	Left 5' - TTCAGACAACGGCAGACAAG Right 5' - TCTGTAGAGGGTTTGGTGGC
Sequence	BJ482431
PCR size	351