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GrainGenes Marker Report: k06632

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Locus
k06632
Type
CAPS
Chromosome
3H
Map
Hv-HNxH602-3H69.2
[ Show Nearby Loci ]
Map Data
Barley, HN x H602, Sato2009
Species
Hordeum vulgare
Hordeum vulgare subsp. spontaneum
Probe
BaAK30M07
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark
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Restriction enzyme used for CAPS analysis = Bsp1286I
SNP = T/C
SNP structure (UNICODE) = CTGTAGTGCTYCTTCGTCAGG
Parent 1 SNP sequence = CTGTAGTGCTTCTTCGTCAGG
Parent 2 SNP sequence = CTGTAGTGCTCCTTCGTCAGG
Recognized sequence for CAPs locus = GDGCHC



Probe for Locus
BaAK30M07
Locus
k06632
Reference
ReferenceSato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks
Best BLAST Hit = metallothioneine type2 [Hordeum vulgare subsp. vulgare]
Type
PCR
PCR primers
Left 5' - TAGATGGCCTCAGTCGGGTA
Right 5' - GAAGCCTCATTCCGTCTTGA
Sequence
AV945803
PCR size
281