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GrainGenes Marker Report: k06586

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Locus	k06586
Type	CAPS
Chromosome	1H
Map	Hv-HNxH602-1H 27.7
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	BaAK21D02
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = AfaI SNP = A/G SNP structure (UNICODE) = CAATGAGGCCRTACAGGGCAA Parent 1 SNP sequence = CAATGAGGCCATACAGGGCAA Parent 2 SNP sequence = CAATGAGGCCGTACAGGGCAA Recognized sequence for CAPs locus = GTAC
Probe for Locus	BaAK21D02
Locus	k06586
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = Vacuolar ATP synthase 16 kDa proteolipid subunit gb AAC12798.1 adenosine triphosphatase; c-subunit of V-ATPase [Vigna radiata]
Type	PCR
PCR primers	Left 5' - AATTACGCAGGGCAACAAAG Right 5' - AAGAGGGTGAGGGAGGATGT
Sequence	BJ461909
PCR size	287