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GrainGenes Marker Report: k06475

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Locus	k06475
Type	CAPS
Chromosome	5H
Map	Hv-HNxH602-5H 20.5
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	BaAK28L16
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = HaeIII SNP = T/G SNP structure (UNICODE) = AGAAGGTTTTKGCCACCGAAG Parent 1 SNP sequence = AGAAGGTTTTTGCCACCGAAG Parent 2 SNP sequence = AGAAGGTTTTGGCCACCGAAG Recognized sequence for CAPs locus = GGCC
Probe for Locus	BaAK28L16
Locus	k06475
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = enolase [Oryza sativa (japonica cultivar-group)]
Type	PCR
PCR primers	Left 5' - ATATCCTGCTGGGCATCATC Right 5' - CCAGCAACAAGGCTTATCGT
Sequence	BQ658903
PCR size	437