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GrainGenes Marker Report: k00535

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Locus	k00535
Type	CAPS
Chromosome	2H
Map	Hv-HNxH602-2H 79.3
	[ Show Nearby Loci ]
Map Data	Barley, HN x H602, Sato2009
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	baak33f06
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
Remark	[ Hide all but 1 of 6 ] Restriction enzyme used for CAPS analysis = HinfI SNP = A/G SNP structure (UNICODE) = ACGTCAAGGGRTTCCATTCTT Parent 1 SNP sequence = ACGTCAAGGGATTCCATTCTT Parent 2 SNP sequence = ACGTCAAGGGGTTCCATTCTT Recognized sequence for CAPs locus = GANTC
Probe for Locus	baak33f06
Locus	k00535
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117.
General Remarks	Best BLAST Hit = plastid omega-3 fatty acid desaturase [Triticum aestivum]
Type	PCR
PCR primers	Left 5' - GACAAAGGCGAGCAGGTTAC Right 5' - GCCAACTTATCCTCCGTTCA
Sequence	BJ458760
PCR size	385