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GrainGenes Marker Report: Xwms6

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Locus	Xwms6
Type	Microsatellite
Chromosome	5R
Chromosome Arm	5RS
Map	Secale-UxE-5 28.7
	[ Show Nearby Loci ]
Map Data	Rye, UC90 x E-line
Probe	WMS6
Remark	(Curator Note, Aug 2019, VCB) locus is missnamed
Possible Orthologs	[ Hide all but 1 of 17 ] WMS6 Xgwm6 Xgwm6-4B Xgwm6-4D.1 Xgwm6-4D.2 Xgwm6-5A Xgwm6.2 Xgwm6a Xgwm6b GWM006 gwm6 wms6.2-3 gwm6-4B wms6.2-3-5A gwm6.1-4B gwm6.2-5A gwm6-5A
Probe for Locus	WMS6
Locus	Xgwm6.1-4B [ Show all 18 ]
Reference	Fu YB et al. (2007) Microsatellite variation in Avena sterilis oat germplasm Theoretical and Applied Genetics 114:1029-1038. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023. Liu Z-W et al. (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theoretical and Applied Genetics 93:869-876. Roeder MS et al. (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246:327-333.
General Remarks	Insert contains repeat (GA)40 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER. Repeat type (GA)x40. Product size 205 bp in Cheyenne. In 369 Avena sterilis accessions there were 3 alleles of size 48-60 bp, PIC = negative.
Type	Genomic PCR SSR
PCR primers	5' CGTATCACCTCCTAGCTAAACTAG 3' 5' AGCCTTATCATGACCCTACCTT 3' CGTATCACCTCCTAGCTAAACTAG AGCCTTATCATGACCCTACCTT
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS6
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring