Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc398

[Submit comment/correction]

Locus	Xwmc398
Type	Microsatellite
Map	Svevo/Ciccio-6B 78.7 Ta-NxW-6B 80.6 Wheat, UC1110 x PI610750 6B 59.7
	[ Show Nearby Loci ]
Breakpoint Interval	6BS5-0.76-1.05
Map Data	Durum wheat, Svevo x Ciccio Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC398
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc398-6A [ Show all 7 ]
Probe for Locus	WMC398
Locus	[ Hide all but 1 of 8 ] Xwmc398b-6A Xwmc398a-6B Xwmc398-6B Xwmc398 Xwmc398-6A wmc398 wmc398-6B wmc398-6A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22c7 Donor: Romestant, RAGT Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 156 bases located on 6B Trimmed to insert at 5' end, corrupted 3' end removed as cleanly as possible. Bases 149 to 198 show homology to 11a8 bases 348 to 397 (50 in longest run, maxScore = 42). (GT)14 32 to 59. Note that the 3' primer is located close to corrupted sequence of vector.
Type	PCR SSR
PCR primers	Forward ggAgATTgAccgAgTggAT at 1 length 19 bases, Tm 60 Reverse cgTgAgAgcggTTcTTTg at 136 length 18 bases, Tm 60
SSR size	152 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC398
Source Species	Triticum aestivum