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GrainGenes Marker Report: Xpsr135-2B

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Locus	Xpsr135-2B
Type	RFLP
Chromosome	2B
Chromosome Arm	2BS
Map	Ta-Courtot/CS-GPW-2B 68.6 Ta-Gale-2B -3.9
	[ Show Nearby Loci ]
Map Data	Wheat, Courtot x Chinese Spring, GPW Wheat, Gale
Species	Triticum aestivum
Probe	PSR135
Reference	Devos KM et al. (1993) Comparative RFLP maps of the homoeologous group-2 chromosomes of wheat, rye and barley Theoretical and Applied Genetics 85:784-792. Sharp PJ et al. (1988) Isolation, characterization and applications of a set of 14 RFLP probes identifying each homoeologous chromosome arm in the Triticeae. Proceedings of the 7th International Wheat Genetics Symposium 639-646.
Data Source	Gale, Michael D. 97.10
Possible Orthologs	psr135 [ Show all 7 ]
Probe for Locus	PSR135
Locus	Xpsr135-2A [ Show all 8 ]
Synonym	Synonym HAR35_14958 HAR35_14959 HAR35_14960
Related Probe	PSR127 PSR182 PSR183 PSR184
	Hybridization_homologue: The two clones were isolated independently but exhibit the same RFLP patterns when hybridized to DNA from test genotypes, and thus are closely related in sequence.
General Remarks	Polymorphism scores are given as the percent polymorphic pairwise comparisons, followed by the number of alleles found and number of varieties tested in parentheses.
Type	cDNA
Sequence	PSR135 Forward PSR135 Reverse
Copy Number	1
Background	3
Cross hybridizes to	Secale cereale good Hordeum vulgare good
Polymorphism	[ Hide all but 1 of 19 ] PSR135 H EcoRI 0.0 (1, 10) PSR135 H EcoRV 0.0 (1, 11) PSR135 H DraI 100.0 (2, 2) PSR135 H HindIII 0.0 (1, 11) PSR135 2B EcoRI 2B 75.2 (5, 15) PSR135 2A EcoRI 2A 51.4 (2, 15) PSR135 2D EcoRI 2D 13.3 (2, 15) PSR135 2B EcoRV 2B 46.7 (4, 15) PSR135 2A EcoRV 2A 0.0 (1, 15) PSR135 2D EcoRV 2D 0.0 (1, 15) PSR135 2A DraI 2A 46.4 (3, 8) PSR135 2D DraI 2D 0.0 (1, 8) PSR135 2B DraI 2B 25.0 (2, 8) PSR135 2B HindIII 2B 13.3 (2, 15) PSR135 2A HindIII 2A 0.0 (1, 15) PSR135 2D HindIII 2D 0.0 (1, 15) PSR135 R EcoRI PSR135 R EcoRV PSR135 R HindIII
Gel	PSR135 Gale
Linkage Group	2AS 2BS 2DS 2H 2S
Insert Enzyme	SmaI
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring
Source Tissue	Leaf
Insert Size	1