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GrainGenes Marker Report: Xgwm540

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Locus	Xgwm540
Type	Microsatellite
Chromosome	5B
Map	Ta-NxW-5B 46.6 Wheat, UC1110 x PI610750 5B 18 Wheat, WH542 x Synthetic 5B 58 Wheat-Composite2004-5B 45 Wheat_Yr79_2018-5BS 37
	[ Show Nearby Loci ]
QTL with Locus Marker	QyrPI182103.wgp-5BS
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750 Wheat, WH542 x Synthetic Wheat_Yr79_2018
Probe	WMS540
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	[ Hide all but 1 of 6 ] Xgwm540-4B Xgwm540-5B Xgwm540a gwm540 gwm540-4B gwm540-5B
Probe for Locus	WMS540
Locus	Xgwm540-5B [ Show all 7 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)3(CC)(CT)16 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCTCGCTGTGAAATCCTATTTC 3' 5' AGGCATGGATAGAGGGGC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS540
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring