GrainGenes Marker Report: Xgwm499
Locus
Xgwm499
Type
Microsatellite
Chromosome
5B
Chromosome Arm
5BL
Map
Svevo/Ciccio-5B 60.5
[ Show all 7 ]
[ Show Nearby Loci ]
Breakpoint Interval
5BL6-0.29-0.79
Map Data
[ Hide all but 1 of 7 ] Durum wheat, Svevo x Ciccio T. turgidum, Langdon x G18-16 Wheat, Composite, 2004 Wheat, Finch x Eltan Wheat, Nanda2419 x Wangshuibai Wheat, WH542 x Synthetic Wheat_Yr79_2018
Probe
WMS499
Possible Orthologs
Xgwm499-5B
[ Show all 6 ]
Probe for Locus
WMS499
Locus
Xgwm499-5B
[ Show all 7 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (GA)32 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' ACTTGTATGCTCCATTGATTGG 3'
5' GGGGAGTGGAAACTGCATAA 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS499
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Xgwm499
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