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GrainGenes Marker Report: Xgwm493

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Locus	Xgwm493
Type	Microsatellite
Chromosome	3B
Chromosome Arm	3BS
Map	Svevo/Ciccio-3B 20.4 [ Show all 6 ]
	[ Show Nearby Loci ]
Breakpoint Interval	3AL5-0.78-1.00
Map Data	[ Hide all but 1 of 6 ] Durum wheat, Svevo x Ciccio T. turgidum, Langdon x G18-16 Wheat, Avalon x Cadenza Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
2 Point Data	Yrns-B1/Xgwm493#1 Distance: 20.5 cM Yrns-B1/Xgwm493#2 Distance: 21.7 cM
Probe	WMS493
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xgwm493-3B Xgwm493-3B.1 Xgwm493-3B.2 gwm493 gwm493-3B
Probe for Locus	WMS493
Locus	Xgwm493-3B [ Show all 7 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)43imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TTCCCATAACTAAAACCGCG 3' 5' GGAACATCATTTCTGGACTTTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS493
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring