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GrainGenes Marker Report: Xgwm264-1B

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Locus	Xgwm264-1B
Type	Microsatellite
Chromosome	1B
Chromosome Arm	1BS
Map	[ Hide all but 1 of 6 ] Chinese_Spring_Deletion_SSR_1B 0.16 Ta-SSR-2004-1B 21.2 Ta-Synthetic/Opata-BARC-1B 11.8 Ta-Synthetic/Opata-GPW-1B 20.2 Ta-Synthetic/Opata-SSR-1B 18.55 Tturgidum-SSR-1B -3.1
	[ Show Nearby Loci ]
QTL with Locus Marker	QLr.pser-1BL
Breakpoint Interval	C-1BL6-0.32
Map Data	T. turgidum, Messapia x dicoccoides, SSR [ Show all 6 ]
2 Point Data	Yr15/Xgwm264
Probe	WMS264
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
Data Source	Roeder, Marion S. 98.09.15
Remark	In Map Ta-Synthetic/Opata-SSR-1B, the intervals most likely to contain locus are Xmwg938 - Xabc156 or Xabc156 - XksuE18
Possible Orthologs	gwm264 [ Show all 14 ]
Probe for Locus	WMS264
Locus	Xgwm264-1B [ Show all 15 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)9A(CA)24 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GAGAAACATGCCGAACAACA 3' 5' GCATGCATGAGAATAGGAACTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS264
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring