GrainGenes Marker Report: Xcfd81-4D
Locus
Xcfd81-4D
Type
Microsatellite
Map
Chinese_Spring_Deletion_SSR_4D 0.155 Ta-Synthetic/Opata-GPW-4D 14.9
[ Show Nearby Loci ]
Breakpoint Interval
C-4DL9-0.31
Map Data
Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe
CFD81
Possible Orthologs
Xcfd81-5D
[ Show all 6 ]
Probe for Locus
CFD81
Locus
[ Hide all but 1 of 7 ] Xcfd81-5D Xcfd81-4D Xcfd81-7B Xcfd81b cfd81 Xcfd81 cfd81-5D
Reference
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GT)14(GA)19 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TATCCCCAATCCCCTCTTTC 3'
Reverse 5' GTCAATTGTGGCTTGTCCCT 3'
SSR size
283
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
7B/5D/4D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd81-4D
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
| ||||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
| |||||||||
|
|
