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GrainGenes Marker Report: Xcfd79.2-3B

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Locus	Xcfd79.2-3B
Type	Microsatellite
Map	P91193D1/P92201D5-3B 29.3
	[ Show Nearby Loci ]
Map Data	Wheat, P91193D1 x P92201D5
Probe	CFD79
Possible Orthologs	cfd79 [ Show all 15 ]
Probe for Locus	CFD79
Locus	[ Hide all but 1 of 16 ] Xcfd79-3B Xcfd79-3D Xcfd79.2-3A Xcfd79.3-3B Xcfd79.2 Xcfd79.1 Xcfd79.2-3B Xcfd79b Xcfd79a Xcfd79-3A Xcfd79-3B.2 Xcfd79-3B.1 cfd79 cfd79-3B Xcfd79 cfd79-3A
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)26 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TCTGGTTCTTGGGAGGAAGA 3' Reverse 5' CATCCAACAATTTGCCCAT 3'
SSR size	284
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	3D/3B
Source Species	Aegilops tauschii