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GrainGenes Marker Report: Xcfd79-3B

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Locus
Xcfd79-3B
Type
Microsatellite
Chromosome
3B
Map
Chinese_Spring_Deletion_SSR_3B-0.165
Conan/Reeder-3B30.0949
Kofa/UC1113-3B22
Ta-Courtot/CS-GPW-3B41.2
Ta-SSR-2004-3B14.5
[ Show Nearby Loci ]
Breakpoint Interval
C-3BS1-0.33
Map Data
Durum wheat, Kofa x UC1113
Wheat, Conan x Reeder
Wheat, Consensus SSR, 2004
Wheat, Courtot x Chinese Spring, GPW
Wheat, Physical, SSR
Probe
CFD79
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
[ Hide all but 1 of 15 ]
cfd79
Xcfd79-3A
Xcfd79-3B.1
Xcfd79-3B.2
Xcfd79-3D
Xcfd79.1
Xcfd79.2
Xcfd79.2-3A
Xcfd79.2-3B
Xcfd79.3-3B
Xcfd79a
Xcfd79b
cfd79-3B
Xcfd79
cfd79-3A



Probe for Locus
CFD79
Locus
Xcfd79-3B
[ Show all 16 ]
Reference
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = D
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (GA)26
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' TCTGGTTCTTGGGAGGAAGA 3'
Reverse 5' CATCCAACAATTTGCCCAT 3'
SSR size
284
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3D/3B
Source Species
Aegilops tauschii