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GrainGenes Marker Report: Xcfd76-6D

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Locus	Xcfd76-6D
Type	Microsatellite
Chromosome	6D
Map	[ Hide all but 1 of 6 ] Chinese_Spring_Deletion_SSR_6D 0.38 CS/SQ1-6D 104.4 Louise/Penawawa-6D 118.5 P91193D1/P92201D5-6D 62.2 Ta-Courtot/CS-GPW-6D 45.3 Ta-SSR-2004-6D 61.2
	[ Show Nearby Loci ]
Breakpoint Interval	6DL6-0.29-0.47
Map Data	Wheat, Chinese Spring x SQ1 [ Show all 6 ]
Probe	CFD76
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xcfd76 cfd76-6D cfd76
Probe for Locus	CFD76
Locus	Xcfd76-6D Xcfd76 cfd76-6D cfd76
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)26 Regularity of motif is imperfect
Type	PCR SSR
PCR primers	Forward 5' GCAATTTCACACGCGACTTA 3' Reverse 5' CGCTCGACAACATGACACTT 3'
SSR size	166
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	6D
Source Species	Aegilops tauschii