GrainGenes Marker Report: Xcfd7-5D
Locus
Xcfd7-5D
Type
Microsatellite
Chromosome
5D
Chromosome Arm
5DL
Map
Chinese_Spring_Deletion_SSR_5D 0.67
[ Show all 7 ]
[ Show Nearby Loci ]
Breakpoint Interval
5DL1-0.60-0.74
Map Data
Wheat, Arina x Forno
[ Show all 7 ]
Species
Triticum aestivum
Probe
CFD7
Reference
Possible Orthologs
[ Hide all but 1 of 7 ] Xcfd7 Xcfd7-5B Xcfd7b Xcfd7b-5B cfd7-5D cfd7 cfd7-5B
Probe for Locus
CFD7
Locus
Xcfd7-5D
[ Show all 8 ]
Reference
Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TC)27 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' AGCTACCAGCCTAGCAGCAG 3'
Reverse 5' TCAGACACGTCTCCTGACAAA 3'
SSR size
250
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
5D/5B
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd7-5D
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