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GrainGenes Marker Report: Xcfd62.1-2D

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Locus
Xcfd62.1-2D
Type
Microsatellite
Map
Conan/Reeder-2D116.046
[ Show Nearby Loci ]
Map Data
Wheat, Conan x Reeder
Probe
CFD62
Possible Orthologs
[ Hide all but 1 of 12 ]
Xcfd62
Xcfd62-2D
Xcfd62-3A
Xcfd62-3D
Xcfd62-7A
Xcfd62.1
Xcfd62.2
Xcfd62.2-3D
Xcfd62a
cfd62-3D
cfd62
cfd62-2D



Probe for Locus
CFD62
Locus
Xcfd62.1-2D
[ Show all 13 ]
Reference
ReferenceGuyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B
Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems)
Amplifies motif: (CT)27
Regularity of motif is perfect
Type
PCR
SSR
PCR primers
Forward 5' CAAGAGCTGACCAATGTGGA 3'
Reverse 5' ACGGCGGTGAGATGAG 3'
SSR size
220
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
2D/7A
Source Species
Aegilops tauschii