GrainGenes Marker Report: Xcfd4-3D
Locus
Xcfd4-3D
Type
Microsatellite
Chromosome
3D
Chromosome Arm
3DL
Map
Chinese_Spring_Deletion_SSR_3D 0.135 P91193D1/P92201D5-3D 46.3 Ta-SSR-2004-3D 34 Ta-Synthetic/Opata-GPW-3D 77.8 Triticum-AxF-3D 77.3
[ Show Nearby Loci ]
Breakpoint Interval
C-3DL2-0.27
Map Data
Wheat, Arina x Forno Wheat, Consensus SSR, 2004 Wheat, P91193D1 x P92201D5 Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Species
Triticum aestivum
Probe
CFD4
Reference
Possible Orthologs
[ Hide all but 1 of 8 ] cfd4 Xcfd4 Xcfd4-3B Xcfd4.1 Xcfd4.2 Xcfd4a cfd4-3B Xcfd004
Probe for Locus
CFD4
Locus
Xcfd4-3B
[ Show all 9 ]
Reference
Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (TC)37 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TGCTCCGTCTCCGAGTAGAT 3'
Reverse 5' GGGAAGGAGAGATGGGAAAC 3'
SSR size
262
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
3D/3B
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd4-3D
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