GrainGenes Marker Report: Xcfd39-5A
Locus
Xcfd39-5A
Type
Microsatellite
Chromosome
5A
Map
Chinese_Spring_Deletion_SSR_5A 0.935 T.b.xT.m.-5A 201.4 Ta-SSR-2004-5A 142 Ta-Synthetic/Opata-GPW-5A 152.8 Wheat_Brittle_culm_2013-5A 0
[ Show Nearby Loci ]
Breakpoint Interval
5AL23-0.87-1.00
Map Data
T. boeoticum x monococcum Wheat, 2013, Brittle culm Wheat, Consensus SSR, 2004 Wheat, Physical, SSR Wheat, Synthetic x Opata, GPW
Probe
CFD39
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
[ Hide all but 1 of 13 ] Xcfd39 Xcfd39-4B Xcfd39-4D Xcfd39a Xcfd39b cfd39 cfd39-1 cfd39-4B cfd39-1-5A cfd39-5A cfd39-4D cfd39-5A.1 cfd39-5A.2
Probe for Locus
CFD39
Locus
Xcfd39-4B
[ Show all 14 ]
Reference
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)26 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' CCACAGCTACATCATCTTTCCTT 3'
Reverse 5' CAAAGTTTGAACAGCAGCCA 3'
SSR size
175
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
4B/5A/4D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd39-5A
|
| |||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
| ||||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
| ||||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
|
| |||||||||||||||
|
|