GrainGenes Marker Report: Xcfd30-1A
Locus
Xcfd30-1A
Type
Microsatellite
Chromosome
1A
Map
Ta-SSR-2004-1A 57.7
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
CFD30
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
[ Hide all but 1 of 7 ] Xcfd30 Xcfd30-4A Xcfd30-6A Xcfd30-7D cfd30 cfd30-1A cfd30-4A
Probe for Locus
CFD30
Locus
Xcfd30-6A
[ Show all 9 ]
Reference
General Remarks
Quality Score = D Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)33(GCA)4 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' AATCGCACAACAATGGTTCA 3'
Reverse 5' GCCTCTCCTCTCTGCTCCTT 3'
SSR size
236
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
6A
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd30-1A
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