GrainGenes Marker Report: Xcfd26
Locus
Xcfd26
Type
Microsatellite
Chromosome
5D
Map
Wheat-Composite2004-5D 48
[ Show Nearby Loci ]
Map Data
Wheat, Composite, 2004
Probe
CFD26
Possible Orthologs
Xcfd26-2A Xcfd26-5D Xcfd26-7D cfd26 cfd26-5D
Probe for Locus
CFD26
Locus
[ Hide all but 1 of 7 ] Xcfd26-5D Xcfd26 Xcfd26-7D Xcfd26-2A Pm35 cfd26 cfd26-5D
Reference
General Remarks
Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GAGAA)2(GA)37 Regularity of motif is perfect
Type
PCR SSR
PCR primers
Forward 5' TCAAGATCGTGCCAAATCAA 3'
Reverse 5' ACTCCAAGCTGAGCACGTTT 3'
SSR size
271
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
5D
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd26
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