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GrainGenes Marker Report: Xcfd21-1D

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Locus	Xcfd21-1D
Type	Microsatellite
Chromosome	1D
Map	Chinese_Spring_Deletion_SSR_1D -0.85 Ta-Courtot/CS-GPW-1D 24.4 Ta-SSR-2004-1D 28
	[ Show Nearby Loci ]
Breakpoint Interval	1DS5-0.70-1.00
Map Data	Wheat, Consensus SSR, 2004 Wheat, Courtot x Chinese Spring, GPW Wheat, Physical, SSR
Probe	CFD21
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	[ Hide all but 1 of 14 ] Xcfd21-1A Xcfd21-7D Xcfd21a Xcfd21b cfd21 cfd21_1 cfd21_2 cfd21-1A Xcfd21 cfd21-1D.1 cfd21-1D.2 cfd21-1D.3 cfd21-7D.1 cfd21-7D.2
Probe for Locus	CFD21
Locus	Xcfd21-1D [ Show all 15 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = C Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GA)21 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' CCTCCATGTAGGCGGAAATA 3' Reverse 5' TGTGTCCCATTCACTAACCG 3'
SSR size	281
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	7D/1D
Source Species	Aegilops tauschii