GrainGenes Marker Report: Xcfd190-6B
Locus
Xcfd190-6B
Type
Microsatellite
Chromosome
6B
Chromosome Arm
6BS
Map
Triticum-AxF-6B 26.1
[ Show Nearby Loci ]
Map Data
Wheat, Arina x Forno
Species
Triticum aestivum
Probe
CFD190
Reference
Paillard S et al. (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.) Theoretical and Applied Genetics 107:1235-1242.
Possible Orthologs
[ Hide all but 1 of 7 ] Xcfd190 Xcfd190-6A Xcfd0190-6B Xcfd190-6D cfd190 cfd190-6A cfd190-6D
Probe for Locus
CFD190
Locus
Xcfd190-6A
[ Show all 8 ]
Reference
General Remarks
Quality Score = B Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (CA)20 Regularity of motif is imperfect
Type
PCR SSR
PCR primers
Forward 5' CAATCAGAAGCGCCATTGTT 3'
Reverse 5' CCCTGATGTTTTCTTTTTCTCC 3'
SSR size
191
Amplification Conditions
5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group
6A
Source Species
Aegilops tauschii
GrainGenes Marker Report: Xcfd190-6B
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